Cleavable detergent

PPS Silent SurfactantProteasMAX
Cleavable detergents, also known as cleavable surfactants, are special surfactants (detergents) that are used in biochemistry and especially in proteomics to enhance protein denaturation and solubility.wikipedia
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Surfactant

surfactantswetting agentbiosurfactant
Cleavable detergents, also known as cleavable surfactants, are special surfactants (detergents) that are used in biochemistry and especially in proteomics to enhance protein denaturation and solubility.

In-gel digestion

in gel
Applications for cleavable detergents include protease digestion of proteins such as in-gel digestion with trypsin after SDS PAGE and peptide extractions from electrophoresis gels.
Cleavable detergents are detergents that are cleaved after digestion, often under acidic conditions.

1-(Furan-2-yl)undecan-1-ol

This cleavable detergent is sensitive to heat and acid and is degraded during a typical trypsin digestion into the uncharged lipophilic compound 1-(furan-2-yl)undecan-1-ol and the zwitterionic 3-aminopropane-1-sulfonic acid (homotaurine), which can be removed by C18 solid phase extraction during sample work-up.
1-(Furan-2-yl)undecan-1-ol is an uncharged lipophilic degradation product of the surfactant ProteasMAX.

Biochemistry

biochemistbiochemicalbiological chemistry
Cleavable detergents, also known as cleavable surfactants, are special surfactants (detergents) that are used in biochemistry and especially in proteomics to enhance protein denaturation and solubility.

Proteomics

proteomicprotein analysisHuman Proteome Resource
Cleavable detergents, also known as cleavable surfactants, are special surfactants (detergents) that are used in biochemistry and especially in proteomics to enhance protein denaturation and solubility.

Protein

proteinsproteinaceousstructural proteins
Cleavable detergents, also known as cleavable surfactants, are special surfactants (detergents) that are used in biochemistry and especially in proteomics to enhance protein denaturation and solubility. Applications for cleavable detergents include protease digestion of proteins such as in-gel digestion with trypsin after SDS PAGE and peptide extractions from electrophoresis gels.

Denaturation (biochemistry)

denaturationdenatureddenature
Cleavable detergents, also known as cleavable surfactants, are special surfactants (detergents) that are used in biochemistry and especially in proteomics to enhance protein denaturation and solubility.

Acid

acidicacidityacids
This cleavable detergent is sensitive to heat and acid and is degraded during a typical trypsin digestion into the uncharged lipophilic compound 1-(furan-2-yl)undecan-1-ol and the zwitterionic 3-aminopropane-1-sulfonic acid (homotaurine), which can be removed by C18 solid phase extraction during sample work-up. The detergent is rendered inactive by cleavage, usually under acidic conditions, in order to make the sample compatible with a following procedure or in order to selectively remove the cleavage products.

Protease

proteasespeptidaseproteinase
Applications for cleavable detergents include protease digestion of proteins such as in-gel digestion with trypsin after SDS PAGE and peptide extractions from electrophoresis gels.

Digestion

digestivedigestdigested
Applications for cleavable detergents include protease digestion of proteins such as in-gel digestion with trypsin after SDS PAGE and peptide extractions from electrophoresis gels.

Trypsin

trypticanti-tryptictrypsin serine proteases
Applications for cleavable detergents include protease digestion of proteins such as in-gel digestion with trypsin after SDS PAGE and peptide extractions from electrophoresis gels.

SDS-PAGE

SDS-polyacrylamide gel electrophoresisSDS PAGEpolyacrylamide gel electrophoresis
Applications for cleavable detergents include protease digestion of proteins such as in-gel digestion with trypsin after SDS PAGE and peptide extractions from electrophoresis gels.

Peptide

polypeptidepeptidespolypeptides
Applications for cleavable detergents include protease digestion of proteins such as in-gel digestion with trypsin after SDS PAGE and peptide extractions from electrophoresis gels.

Electrophoresis

electrophoreticelectrophoretic mobilitymobility
Applications for cleavable detergents include protease digestion of proteins such as in-gel digestion with trypsin after SDS PAGE and peptide extractions from electrophoresis gels.

Mass spectrometry

mass spectrometerMSmass spectrometric
Cleavable detergents are mainly used in sample preparations for mass spectrometry.

Acetal

ketalacetalsacetalisation
This acetalic detergent is split under acidic conditions into hexanol and the zwitterionic 3-acetyl-1-(3-sulfopropyl)pyridinium. MALDI matrix compounds such as α-cyano-4-hydroxycinnamic acid have been linked through a linker consisting of an unsymmetric formaldehyde acetals to dodecanol.

Hexanol

4%Hx
This acetalic detergent is split under acidic conditions into hexanol and the zwitterionic 3-acetyl-1-(3-sulfopropyl)pyridinium.

Zwitterion

zwitterionicZwitterionsinner salt
This cleavable detergent is sensitive to heat and acid and is degraded during a typical trypsin digestion into the uncharged lipophilic compound 1-(furan-2-yl)undecan-1-ol and the zwitterionic 3-aminopropane-1-sulfonic acid (homotaurine), which can be removed by C18 solid phase extraction during sample work-up. This acetalic detergent is split under acidic conditions into hexanol and the zwitterionic 3-acetyl-1-(3-sulfopropyl)pyridinium.

Promega

Promega BeneluxPromega Corporation
ProteaseMAX'is the brandname of Promega for sodium 3-((1-(furan-2-yl)undecyloxy)carbonylamino)propane-1-sulfonate.

Lipophilicity

lipophilicfat-solublelipid-soluble
This cleavable detergent is sensitive to heat and acid and is degraded during a typical trypsin digestion into the uncharged lipophilic compound 1-(furan-2-yl)undecan-1-ol and the zwitterionic 3-aminopropane-1-sulfonic acid (homotaurine), which can be removed by C18 solid phase extraction during sample work-up.

Homotaurine

tramiprosate3-aminopropane-1-sulfonic acidHomotaurine (tramiprosate)
This cleavable detergent is sensitive to heat and acid and is degraded during a typical trypsin digestion into the uncharged lipophilic compound 1-(furan-2-yl)undecan-1-ol and the zwitterionic 3-aminopropane-1-sulfonic acid (homotaurine), which can be removed by C18 solid phase extraction during sample work-up.

Solid phase extraction

extractionSolid-phase extractionMicroextraction by Packed Sorbent
This cleavable detergent is sensitive to heat and acid and is degraded during a typical trypsin digestion into the uncharged lipophilic compound 1-(furan-2-yl)undecan-1-ol and the zwitterionic 3-aminopropane-1-sulfonic acid (homotaurine), which can be removed by C18 solid phase extraction during sample work-up.

Waters Corporation

WatersJames Logan WatersMicromass
RapiGest SF, the brand-name for sodium 3-[(2-methyl-2-undecyl-1,3-dioxolan-4-yl)methoxy]-1-propanesulfonate, is an acid-cleavable anionic detergent marketed by Waters Corporation and AOBIOUS INC.

Alpha-Cyano-4-hydroxycinnamic acid

α-Cyano-4-hydroxycinnamic acid
MALDI matrix compounds such as α-cyano-4-hydroxycinnamic acid have been linked through a linker consisting of an unsymmetric formaldehyde acetals to dodecanol.

Formaldehyde

formalinH 2 COmethanal
MALDI matrix compounds such as α-cyano-4-hydroxycinnamic acid have been linked through a linker consisting of an unsymmetric formaldehyde acetals to dodecanol.