Wright's stain

Wright stainWright-Giemsa stainWrightMay Grunwald-Giemsa methodWright-Giemsa-stainedWright-stained
Wright's stain is a histologic stain that facilitates the differentiation of blood cell types.wikipedia
45 Related Articles

Blood film

blood smearperipheral blood smearblood smears
It is used primarily to stain peripheral blood smears, urine samples, and bone marrow aspirates which are examined under a light microscope.
Routine analysis of blood in medical laboratories is usually performed on blood films stained with Romanowsky stains such as Wright's stain, Giemsa stain, or Diff-Quik.

Romanowsky stain

azure stainsRomanowsky methodAzure 1
It is named for James Homer Wright, who devised the stain, a modification of the Romanowsky stain, in 1902.
Stains that are related to or derived from the Romanowsky-type stains include Giemsa, Jenner, Wright, Field, May–Grünwald stain and Leishman stains.

Methylene blue

Methylthioninium chloride
It is classically a mixture of eosin (red) and methylene blue dyes. The related stains are known as the buffered Wright stain, the Wright-Giemsa stain (a combination of Wright and Giemsa stains), and the buffered Wright-Giemsa stain, and specific instructions depend on the solutions being used, which may include eosin Y, azure B, and methylene blue (some commercial preparations combine solutions to simplify staining).
In biology methylene blue is used as a dye for a number of different staining procedures, such as Wright's stain and Jenner's stain.

Giemsa stain

GiemsaGiemsa stainingGiemsa-stained
The related stains are known as the buffered Wright stain, the Wright-Giemsa stain (a combination of Wright and Giemsa stains), and the buffered Wright-Giemsa stain, and specific instructions depend on the solutions being used, which may include eosin Y, azure B, and methylene blue (some commercial preparations combine solutions to simplify staining).
Giemsa stain is also a differential stain, such as when it is combined with Wright stain to form Wright-Giemsa stain.

James Homer Wright

James WrightWright
It is named for James Homer Wright, who devised the stain, a modification of the Romanowsky stain, in 1902.
He is the "Wright" in Wright's stain, and the "Homer Wright rosettes" associated with neuroblastoma.

Diff-Quik

The Diff-Quik procedure is based on a modification of the Wright-Giemsa stain pioneered by Harleco in the 1970s, and has advantages over the routine Wright-Giemsa staining technique in that it reduces the 4-minute process into a much shorter operation and allows for selective increased eosinophilic or basophilic staining depending upon the time the smear is left in the staining solutions.

Staining

stainstainedstains
Common variants include Wright's stain, Jenner's stain, May-Grunwald stain, Leishman stain and Giemsa stain.

Leishman stain

Leishman's stainLeishman
It is a version of the Romanowsky stain, and is thus similar to and partially replaceable by Giemsa stain, Jenner's stain, and Wright's stain.

Histology

histologicalhistologichistologist
Wright's stain is a histologic stain that facilitates the differentiation of blood cell types.

Blood cell

blood cellshematopoietic cellhemocyte
Wright's stain is a histologic stain that facilitates the differentiation of blood cell types.

Eosin

eosine yellowish-(ys)bromo acidseosin y
It is classically a mixture of eosin (red) and methylene blue dyes.

Bone marrow

marrowred bone marrowbone marrow stroma
It is used primarily to stain peripheral blood smears, urine samples, and bone marrow aspirates which are examined under a light microscope.

Fine-needle aspiration

fine needle aspirationneedle aspiration biopsyneedle biopsy
It is used primarily to stain peripheral blood smears, urine samples, and bone marrow aspirates which are examined under a light microscope.

Optical microscope

light microscopeoptical microscopycompound microscope
It is used primarily to stain peripheral blood smears, urine samples, and bone marrow aspirates which are examined under a light microscope.

Cytogenetics

cytogeneticcytogeneticistchromosome analysis
In cytogenetics, it is used to stain chromosomes to facilitate diagnosis of syndromes and diseases.

White blood cell

leukocyteleukocyteswhite blood cells
Because it distinguishes easily between blood cells, it became widely used for performing differential white blood cell counts, which are routinely ordered when conditions such as infection or leukemia are suspected.

Eosin Y

The related stains are known as the buffered Wright stain, the Wright-Giemsa stain (a combination of Wright and Giemsa stains), and the buffered Wright-Giemsa stain, and specific instructions depend on the solutions being used, which may include eosin Y, azure B, and methylene blue (some commercial preparations combine solutions to simplify staining).

May–Grünwald stain

The May–Grünwald stain, which produces a more intense coloration, also takes a longer time to perform.

Eosinophil

eosinophilseosinophil granulocyteEosino
Urine samples stained with Wright's stain will identify eosinophils, which can indicate interstitial nephritis or urinary tract infection.

Interstitial nephritis

tubulointerstitial nephritisacute interstitial nephritisTubulo-interstitial nephritis
Urine samples stained with Wright's stain will identify eosinophils, which can indicate interstitial nephritis or urinary tract infection.

Urinary tract infection

cystitisurinary tract infectionsbladder infection
Urine samples stained with Wright's stain will identify eosinophils, which can indicate interstitial nephritis or urinary tract infection.

Critical green inclusion

They appear brightly coloured and refractile when stained with Wright-Giemsa stain.

Borrelia turicatae

B. turicatae is best seen by dark-field microscopy, but the organisms can also be detected using acridine orange or Wright's stain.

Echinocyte

Echinocytes
Echinocytes also exhibit central pallor, or lightening of color in the center of the cell under Wright staining.